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This is the wiki for Genome Analysis project.

The aim of this project is to replicate the results from the article "Multi-omics Reveals the Lifestyle of the Acidophilic, Mineral-Oxidizing Model Species Leptospirillum ferriphilum" by Christel et al.

There are two sets of data available from the article, which are PacBio reads of DNA and Illumina reads of RNA under two different growth conditions. These data would be the starting point of the project. All the analysis in the project is carried out the the above data. The DNA data is used to assemble the genome and to annotate genes present in them. This is then used to map against the transcriptome data to find the proteins responsible for the metabolism in the organism. Data Two sets of data were used for the analysis. The first dataset is several files of DNA reads resulting from the PacBio sequencing of the L. ferriphilum genome. This data will be used to assemble and annotate the genome of the bacterium. The second dataset is Illumina RNA sequencing data from L. ferriphilum grown under the two different conditions described above. This data will be used to run a differential expression analysis and get an expression profile for each condition.