General Image Fiber Tool (GIFT) is an ImageJ macro tool which allows the users to measure the average diameter of electrospun fibers in scanning electron microscopy (SEM) images. The theory behind GIFT and the development into a the available macro is thoroughly explained and validated in our previously published works (Götz et al, 2021. https://doi.org/10.1016/j.measurement.2021.109265 and Huling et al, 2022. https://doi.org/10.1371/journal.pone.0275528 ).
Measuring fiber diameters as an experimental endpoint or as a means of quality control is an important step in the fabrication of nonwoven materials. GIFT allows for the quick and reliable batch analysis of large sets of images and allows users control over the image analysis parameters. Results are presented in summarized or raw data formats. GIFT may also be applied to non-electrospinning images, and examples are given in the supplemental info section of Götz et al 2021.
Please credit and cite the following papers when using GIFT:
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A. Götz, V. Senz, W. Schmidt, J. Huling, N. Grabow, S. Illner, General image fiber tool: A concept for automated evaluation of fiber diameters in SEM images, Measurement 177 (2021) 109265. https://doi.org/10.1016/j.measurement.2021.109265.
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Huling J, Götz A, Grabow N, Illner S (2022) GIFT: An ImageJ macro for automated fiber diameter quantification. PLoS ONE 17(10): e0275528. https://doi.org/10.1371/journal.pone.0275528
Instructions for installation and use, as well as trouble shooting tips and examples are in the User Guide. SEM images used in the 2022 manuscript are in the Examples Images folder.