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DavidAlberto/README.md

👋 Hi, I'm David Alberto García Estrada

Pharmaceutical Chemist | Biologist | Bioinformatics Researcher
PhD Candidate in Integrative Biology at Cinvestav · Research Assistant, Laboratorio de Nanotoxicología y Genómica Funcional, CIMAV
🔬 Multi-omics meets fungal biology: decoding cytokinin signaling in Trichoderma atroviride

🌐 Portafolio y CV completo →

"Pursue excellence, and success will follow."


🧠 Research Interests

I work at the intersection of experimental biology, bioinformatics, and data science, specializing in:

  • 🌱 Fungal Development & Hormone Signaling
    Multi-omics analysis of cytokinin signaling during fungal germination and morphogenesis.

  • 🧬 Functional & Comparative Genomics
    Mining gene families and adaptive traits in fungi and extremophiles.

  • 🌿 Microbial Ecology & Metagenomics
    Profiling soil and gut microbiomes using high-throughput sequencing and diversity metrics.

  • 🔁 Reproducible Data Science
    Developing pipelines for RNA-seq, metagenomics, and genome annotation.


🔬 Current Research Projects

  • Cytokinin-responsive transcriptomics in T. atroviride (Cinvestav)
  • Complete high-quality genome of T. atroviride IMI206040 — secondary metabolism and lncRNA regulation during development (Cinvestav)
  • Nanotoxicology of iron oxide nanoparticles in filamentous fungi and frijol pinto menonita (Phaseolus vulgaris) — tripartite plant–nanoparticle–fungus interactions in the context of arsenic/fluoride remediation (CIMAV, MCyTA program)
  • BioCode Academy
    📘 Bioinformatics Education Platform — Plataforma educativa dedicada a la formación en bioinformática, desde conceptos básicos hasta análisis avanzados

🤝 Collaborations

  • 🧄 Stromatinia cepivora Transcriptomics – Universidad de Guanajuato
    Dra. Patricia Ponce Noyola & Dr. Alberto Flores Martínez

  • 🧬 CAMDA 2024/2025: Gut Microbiome Challenge – UNAM
    Dra. Nelly Sélem Mojica & Dr. Shaday Guerrero Flores

  • 🌾 MicroAgroBiome 2.0: atlas evolutivo del metabolismo especializado en microbiomas de cultivos – CCM (UNAM) & Centro de Biotecnología FEMSA (Tec de Monterrey)
    Dra. Adriana Haydeé Contreras Peruyero

  • 🔬 Genome Mining of Prauserella species – CCM (UNAM) & Universidad de Sevilla
    Dra. Nelly Sélem Mojica & Dra. Paulina Corral Villa


🛠️ Technical Skills

💻 Bioinformatics & Data Science

Genomics Transcriptomics Metagenomics Genome Mining Protein modeling Sequence analysis Phylogenetic analysis BLAST GATK R Bioconductor Python Shell Git/GitHub Docker

🔬 Wet Lab Techniques

Fungal Genetics PCR RT-PCR qPCR dPCR Microscopy Protein Purification Cloning Heterologous Expression Antibody Production


📚 Education

  • 🎓 PhD in Integrative Biology – Cinvestav (en proceso de titulación)
    Cytokinin signaling in T. atroviride, Dr. Alfredo Herrera Estrella's lab

  • 🎓 MSc in Biological Sciences – Universidad de Guanajuato
    Transcriptomics in Sclerotium cepivorum, graduated with highest honors

  • 🎓 BSc in Pharmaceutical Chemistry and Biology – Universidad de Guanajuato
    Graduated with distinction


🧑‍🏫 Teaching & Outreach

  • 🎓 Mentor and instructor in molecular biology, chemistry, and bioinformatics
  • 📘 Taller de Análisis de Secuencias y Modelado Proteíco — Programa de Maestría y Doctorado, Universidad de Guanajuato (2025)
  • 📘 Workshop co-instructor in Transcriptomic, Metagenomic, Genome mining, and statistics with R
  • 🧪 Active contributor to academic seminars and open-science education

📈 GitHub Stats

David's GitHub stats


📰 Publications

  • García-Estrada et al. (2024)
    Diversity of bacterial communities in wetlands of Calakmul Biosphere Reserve: a comparative analysis between conserved and semi-urbanized zones in pre-Mayan Train era.
    BMC Microbiology 24, 376

  • Aguilar, Fontove-Herrera, Pashkov, García-Estrada et al. (2026)
    MicroAgroBiome: a toolkit for exploring specialized metabolism and ecological interactions in rhizosphere microbiomes of cultivated crops.
    Nucleic Acids Research, 54(D1), D1743–D1752.

  • Hernández-Figueroa, Herrera-Pérez, García-Estrada et al. (2026)
    Nanopriming with Zn-doped MgO nanoparticles improves germination associated with redox homeostasis in common bean (Phaseolus vulgaris L.).
    Environmental Science: Nano.

  • García-Estrada, Ruvalcaba-Villagrán, Sánchez-Fonseca et al. (2026)
    Phytohormones in fungi: inter-kingdom modulators or fungal self-controlling elements?
    Microbiology and Molecular Biology Reviews, e00261-25.


📬 Contact


🧘‍♂️ Fun Facts

  • 🪵 Weekend woodworker
  • 🍳 Experimental chef

Popular repositories Loading

  1. Calakmul Calakmul Public

    This repository includes all the scripts used for metagenomic analysis of data obtained from the Calakmul Biosphere Reserve.

    1

  2. Phytohormones-Fungi Phytohormones-Fungi Public

    This repository describes a reproducible bioinformatics workflow designed to identify, curate, and analyze homologs of phytohormone-associated genes in filamentous fungi.

    Shell 1

  3. FindFungi FindFungi Public

    FindFungi es un pipeline que permite la búsqueda de hongos presentes en muestras metagenómicas de Shotgun. El siguiente pipeline es una modificación del desarrollado por Donovan y col., 2018.

    Python

  4. DavidAlberto DavidAlberto Public

  5. CAMDA2024 CAMDA2024 Public

    Diversity Analysis

  6. dashboard-rhizosphere dashboard-rhizosphere Public