Error: BLAST tabular options (-o) have not been set.

[desmodus1984]

Hi,

I followed the instructions also as stated here:
https://github.com/wurmlab/genevalidator/wiki/Setting-Up-BLAST-Databases

and run failed.

This is the code I ran:

blastp \
 -max_target_seqs 10 \
 -num_threads 100 \
 -query /data/common/juanpablo.aguilar/data/genomes/ortho-5/erroz/Lepwed.pep \
 -outfmt '7 qseqid sseqid sacc slen qstart qend sstart send length qframe pident nident evalue qseq sseq' \
 -db uniprot_sprot \
 -evalue 0.000001 \
 -out LW_blast.tsv

/data/common/juanpablo.aguilar/apps/genevalidator-2.1.12-linux-x86_64/genevalidator -n 100 -t LW_blast.tsv \
        -o 'qseqid sseqid sacc slen qstart qend sstart send length qframe pident nident evalue qseq sseq' \
        /data/common/juanpablo.aguilar/data/genomes/ortho-5/erroz/Lepwed.pep

and the error:

==> Analysing input arguments
*** Error: BLAST tabular options (-o) have not been set.
    Please set the "-o" option with the custom format
    used in the BLAST -outfmt argument