가장 상세한 워크플로를 가진 'Biosensor_library_construction' 실험을 기준으로 생성되었습니다.
graph LR
subgraph wf0 ["WB000 Material Preparation - Goldengate_assembly_part_preparation"]
uo_0_0["UH400<br/>Manual\"]
end
subgraph wf1 ["WB030 DNA Assembly - Level_0_to_1_Goldengate_assembly"]
uo_1_0["UHW255<br/>Centrifuge"]
uo_1_1["UHW030<br/>Nanoliter Liquid Dispensing"]
uo_1_2["UHW255<br/>Centrifuge"]
uo_1_3["UHW130<br/>Sealing"]
uo_1_4["UHW100<br/>Thermocycling"]
uo_1_0 --> uo_1_1 --> uo_1_2 --> uo_1_3 --> uo_1_4
end
subgraph wf2 ["WB150 PCR-based Target Amplification - TU_amplification_for_level_2_goldengate_assembly"]
uo_2_0["UHW140<br/>Peeling"]
uo_2_1["UHW010<br/>Liquid Handling"]
uo_2_2["UHW010<br/>Liquid Handling"]
uo_2_3["UHW255<br/>Centrifuge"]
uo_2_4["UHW030<br/>Nanoliter Liquid Dispensing"]
uo_2_5["UHW130<br/>Sealing"]
uo_2_6["UHW100<br/>Thermocycling"]
uo_2_0 --> uo_2_1 --> uo_2_2 --> uo_2_3 --> uo_2_4 --> uo_2_5 --> uo_2_6
end
subgraph wf3 ["WB040 DNA Purification - Size-selection_bead-based_DNA_purification"]
uo_3_0["UHW140<br/>Peeling"]
uo_3_1["UHW250<br/>Nucleic Acid Purification"]
uo_3_2["UHW010<br/>Liquid Handling"]
uo_3_3["UHW380<br/>Microplate Reading"]
uo_3_4["UHW010<br/>Liquid Handling"]
uo_3_5["UHW230<br/>Nucleic Acid Fragment Analysis"]
uo_3_0 --> uo_3_1 --> uo_3_2 --> uo_3_3 --> uo_3_4 --> uo_3_5
end
subgraph wf4 ["WB030 DNA Assembly - Level_1_to_2_Goldengate_assembly"]
uo_4_0["UHW255<br/>Centrifuge"]
uo_4_1["UHW130<br/>Sealing"]
uo_4_2["UHW100<br/>Thermocycling"]
uo_4_0 --> uo_4_1 --> uo_4_2
end
subgraph wf5 ["WB120 Biology-mediated DNA Transfers - Transformation_using_Ecoli"]
uo_5_0["UHW140<br/>Peeling"]
uo_5_1["UHW020<br/>96 Channel Liquid Handling"]
uo_5_0 --> uo_5_1
end
subgraph wf6 ["WB130 Solid Media Cell Culture - Spotted_plate_incubation"]
uo_6_0["UHW180<br/>Incubation"]
end
subgraph wf7 ["WB125 Colony Picking - Candidate_colony_picking"]
uo_7_0["UHW060<br/>Colony Picking"]
end
subgraph wf8 ["WB140 Liquid Media Cell Culture - Candidate colony incubation"]
uo_8_0["UHW180<br/>Incubation"]
uo_8_1["UHW010<br/>Liquid Handling"]
uo_8_0 --> uo_8_1
end
subgraph wf9 ["WB045 DNA Extraction - Plate-based_plasmid_prep"]
end
subgraph wf10 ["WB025 Sequencing Library Preparation - NGS_library_preparation_for_long-read_sequencing"]
uo_10_0["UH010<br/>Liquid Handling"]
uo_10_1["UH380<br/>Microplate Reading"]
uo_10_0 --> uo_10_1
end
subgraph wf11 ["WT010 Nucleotide Sequencing - Nanopore_long-read_sequencing"]
end
subgraph wf12 ["WL010 Sequence Variant Analysis - Map_the_reads_to_the_reference"]
uo_12_0["USW120<br/>Sequence Trimming and Filtering"]
uo_12_1["USW130<br/>Read Mapping and Alignment"]
uo_12_2["USW170<br/>Variant Calling"]
uo_12_3["USW340<br/>Computation"]
uo_12_0 --> uo_12_1 --> uo_12_2 --> uo_12_3
end
wf0 --> wf1 --> wf2 --> wf3 --> wf4 --> wf5 --> wf6 --> wf7 --> wf8 --> wf9 --> wf10 --> wf11 --> wf12
gantt
title vector design (001_vector_design)
dateFormat YYYY-MM-DD
axisFormat %m-%d
section WD060 Genetic Circuit Design - rnew
Protein Structure Predict... :crit, USW070, 2025-09-26, 2025-09-26
(표시할 데이터가 없습니다.)
gantt
title Biosensor_library_construction (004_Biosensor_library_construction)
dateFormat YYYY-MM-DD
axisFormat %m-%d
section WB000 Material Preparation - Goldengate_assembly_part_preparation
Manual\ :crit, UH400, 2025-08-20, 2025-09-26
section WB030 DNA Assembly - Level_0_to_1_Goldengate_assembly
Centrifuge :crit, UHW255, 2025-08-14, 2025-08-15
Nanoliter Liquid Dispensing :crit, UHW030, 2025-08-14, 2025-08-15
Centrifuge :crit, UHW255, 2025-08-18, 2025-08-21
Sealing :crit, UHW130, 2025-08-14, 2025-08-15
Thermocycling :crit, UHW100, 2025-08-14, 2025-08-15
section WB150 PCR-based Target Amplification - TU_amplification_for_level_2_goldengate_assembly
Peeling :active, UHW140, 2025-08-14, 2025-09-30
Liquid Handling :active, UHW010, 2025-08-14, 2025-09-30
Liquid Handling :active, UHW010, 2025-08-14, 2025-09-30
Centrifuge :active, UHW255, 2025-08-14, 2025-09-30
Nanoliter Liquid Dispensing :active, UHW030, 2025-08-14, 2025-09-30
Sealing :active, UHW130, 2025-08-14, 2025-09-30
Thermocycling :active, UHW100, 2025-08-14, 2025-09-30
section WB040 DNA Purification - Size-selection_bead-based_DNA_purification
Peeling :active, UHW140, 2025-08-14, 2025-09-30
Nucleic Acid Purification :active, UHW250, 2025-08-14, 2025-09-30
Liquid Handling :active, UHW010, 2025-08-14, 2025-09-30
Microplate Reading :active, UHW380, 2025-08-14, 2025-09-30
Liquid Handling :active, UHW010, 2025-08-14, 2025-09-30
Nucleic Acid Fragment Ana... :active, UHW230, 2025-08-14, 2025-09-30
section WB030 DNA Assembly - Level_1_to_2_Goldengate_assembly
Centrifuge :active, UHW255, 2025-08-18, 2025-09-30
Sealing :active, UHW130, 2025-08-17, 2025-09-30
Thermocycling :active, UHW100, 2025-08-17, 2025-09-30
section WB120 Biology-mediated DNA Transfers - Transformation_using_Ecoli
Peeling :active, UHW140, 2025-08-17, 2025-09-30
96 Channel Liquid Handling :active, UHW020, 2025-08-17, 2025-09-30
section WB130 Solid Media Cell Culture - Spotted_plate_incubation
Incubation :active, UHW180, 2025-08-17, 2025-09-30
section WB125 Colony Picking - Candidate_colony_picking
Colony Picking :active, UHW060, 2025-08-17, 2025-09-30
section WB140 Liquid Media Cell Culture - Candidate colony incubation
Incubation :active, UHW180, 2025-08-17, 2025-09-30
Liquid Handling :active, UHW010, 2025-08-18, 2025-09-30
section WB025 Sequencing Library Preparation - NGS_library_preparation_for_long-read_sequencing
Liquid Handling :active, UH010, 2025-08-14, 2025-09-30
Microplate Reading :active, UH380, 2025-08-14, 2025-09-30
section WL010 Sequence Variant Analysis - Map_the_reads_to_the_reference
Sequence Trimming and Fil... :active, USW120, 2025-08-18, 2025-09-30
Read Mapping and Alignment :active, USW130, 2025-08-18, 2025-09-30
Variant Calling :active, USW170, 2025-08-18, 2025-09-30
Computation :active, USW340, 2025-08-20, 2025-09-30
(표시할 데이터가 없습니다.)