diff --git a/doc/nrf/releases_and_maturity/releases/release-notes-changelog.rst b/doc/nrf/releases_and_maturity/releases/release-notes-changelog.rst
index 736c40c11c5..e9b55cc0118 100644
--- a/doc/nrf/releases_and_maturity/releases/release-notes-changelog.rst
+++ b/doc/nrf/releases_and_maturity/releases/release-notes-changelog.rst
@@ -487,12 +487,6 @@ Bluetooth samples
   The :ref:`ipc_radio` application is commonly used for multicore builds in other |NCS| samples and projects.
   Hence, this is to align with the common practice.
 
-* Removed support for the ``thingy53/nrf5340/cpuapp/ns`` build target from the following samples:
-
-   * :ref:`peripheral_lbs`
-   * :ref:`peripheral_status`
-   * :ref:`peripheral_uart`
-
 * Disabled legacy pairing in the following samples:
 
    * :ref:`central_nfc_pairing`
diff --git a/samples/bluetooth/peripheral_status/boards/thingy53_nrf5340_cpuapp.conf b/samples/bluetooth/peripheral_status/boards/thingy53_nrf5340_cpuapp.conf
index 137a9a7ff35..6f702c71b37 100644
--- a/samples/bluetooth/peripheral_status/boards/thingy53_nrf5340_cpuapp.conf
+++ b/samples/bluetooth/peripheral_status/boards/thingy53_nrf5340_cpuapp.conf
@@ -71,13 +71,13 @@ CONFIG_NORDIC_QSPI_NOR_STACK_WRITE_BUFFER_SIZE=16
 ################################################################################
 # Enable USB CDC ACM
 
-CONFIG_USB_DEVICE_STACK=y
-CONFIG_USB_DEVICE_REMOTE_WAKEUP=n
-CONFIG_USB_DEVICE_PRODUCT="Thingy:53 Application"
-CONFIG_USB_DEVICE_VID=0x1915
-CONFIG_USB_DEVICE_PID=0x530C
-CONFIG_USB_CDC_ACM=y
-CONFIG_USB_DEVICE_LOG_LEVEL_ERR=y
+CONFIG_USB_DEVICE_STACK_NEXT=y
+CONFIG_CDC_ACM_SERIAL_INITIALIZE_AT_BOOT=y
+CONFIG_CDC_ACM_SERIAL_PRODUCT_STRING="Thingy:53 Application"
+CONFIG_CDC_ACM_SERIAL_VID=0x1915
+CONFIG_CDC_ACM_SERIAL_PID=0x530C
+CONFIG_UDC_DRIVER_LOG_LEVEL_ERR=y
+CONFIG_USBD_LOG_LEVEL_ERR=y
 
 # Use CDC_ACM0 for logs
 CONFIG_LOG_BACKEND_UART=y
diff --git a/samples/bluetooth/peripheral_status/boards/thingy53_nrf5340_cpuapp_ns.conf b/samples/bluetooth/peripheral_status/boards/thingy53_nrf5340_cpuapp_ns.conf
index 137a9a7ff35..6f702c71b37 100644
--- a/samples/bluetooth/peripheral_status/boards/thingy53_nrf5340_cpuapp_ns.conf
+++ b/samples/bluetooth/peripheral_status/boards/thingy53_nrf5340_cpuapp_ns.conf
@@ -71,13 +71,13 @@ CONFIG_NORDIC_QSPI_NOR_STACK_WRITE_BUFFER_SIZE=16
 ################################################################################
 # Enable USB CDC ACM
 
-CONFIG_USB_DEVICE_STACK=y
-CONFIG_USB_DEVICE_REMOTE_WAKEUP=n
-CONFIG_USB_DEVICE_PRODUCT="Thingy:53 Application"
-CONFIG_USB_DEVICE_VID=0x1915
-CONFIG_USB_DEVICE_PID=0x530C
-CONFIG_USB_CDC_ACM=y
-CONFIG_USB_DEVICE_LOG_LEVEL_ERR=y
+CONFIG_USB_DEVICE_STACK_NEXT=y
+CONFIG_CDC_ACM_SERIAL_INITIALIZE_AT_BOOT=y
+CONFIG_CDC_ACM_SERIAL_PRODUCT_STRING="Thingy:53 Application"
+CONFIG_CDC_ACM_SERIAL_VID=0x1915
+CONFIG_CDC_ACM_SERIAL_PID=0x530C
+CONFIG_UDC_DRIVER_LOG_LEVEL_ERR=y
+CONFIG_USBD_LOG_LEVEL_ERR=y
 
 # Use CDC_ACM0 for logs
 CONFIG_LOG_BACKEND_UART=y
diff --git a/samples/bluetooth/peripheral_status/sample.yaml b/samples/bluetooth/peripheral_status/sample.yaml
index 56dd10a21b5..a31d6de8f07 100644
--- a/samples/bluetooth/peripheral_status/sample.yaml
+++ b/samples/bluetooth/peripheral_status/sample.yaml
@@ -23,6 +23,7 @@ tests:
       - nrf52840dk/nrf52840
       - nrf5340dk/nrf5340/cpuapp
       - nrf5340dk/nrf5340/cpuapp/ns
+      - thingy53/nrf5340/cpuapp
       - nrf54l15dk/nrf54l05/cpuapp
       - nrf54l15dk/nrf54l10/cpuapp
       - nrf54l15dk/nrf54l15/cpuapp
@@ -34,6 +35,7 @@ tests:
       - nrf52840dk/nrf52840
       - nrf5340dk/nrf5340/cpuapp
       - nrf5340dk/nrf5340/cpuapp/ns
+      - thingy53/nrf5340/cpuapp
       - nrf54l15dk/nrf54l05/cpuapp
       - nrf54l15dk/nrf54l10/cpuapp
       - nrf54l15dk/nrf54l15/cpuapp
@@ -49,6 +51,8 @@ tests:
       - nrf52840dk/nrf52840
       - nrf5340dk/nrf5340/cpuapp
       - nrf5340dk/nrf5340/cpuapp/ns
+      - thingy53/nrf5340/cpuapp
+      - thingy53/nrf5340/cpuapp/ns
       - nrf54l15dk/nrf54l05/cpuapp
       - nrf54l15dk/nrf54l10/cpuapp
       - nrf54l15dk/nrf54l15/cpuapp
@@ -61,6 +65,8 @@ tests:
       - nrf52dk/nrf52810
       - nrf5340dk/nrf5340/cpuapp
       - nrf5340dk/nrf5340/cpuapp/ns
+      - thingy53/nrf5340/cpuapp
+      - thingy53/nrf5340/cpuapp/ns
       - nrf54l15dk/nrf54l05/cpuapp
       - nrf54l15dk/nrf54l10/cpuapp
       - nrf54l15dk/nrf54l15/cpuapp
diff --git a/samples/bluetooth/peripheral_status/sysbuild/mcuboot/boards/thingy53_nrf5340_cpuapp.conf b/samples/bluetooth/peripheral_status/sysbuild/mcuboot/boards/thingy53_nrf5340_cpuapp.conf
index bbef184607e..04524e9ade6 100644
--- a/samples/bluetooth/peripheral_status/sysbuild/mcuboot/boards/thingy53_nrf5340_cpuapp.conf
+++ b/samples/bluetooth/peripheral_status/sysbuild/mcuboot/boards/thingy53_nrf5340_cpuapp.conf
@@ -73,3 +73,6 @@ CONFIG_FLASH_SIMULATOR_STATS=n
 # Enable custom command to erase settings partition.
 CONFIG_ENABLE_MGMT_PERUSER=y
 CONFIG_BOOT_MGMT_CUSTOM_STORAGE_ERASE=y
+
+CONFIG_LTO=y
+CONFIG_ISR_TABLES_LOCAL_DECLARATION=y